How many reactions may be performed in a setup for RT-PCR?

In the context of RT-PCR (reverse transcription-polymerase chain reaction), the setup typically allows for multiple reactions to be performed, especially when analyzing different samples or templates. Each reaction can be tailored to specific targets or conditions.

While it may be feasible to run two reactions in a specific setup with multiplexing techniques, this is somewhat limited and does not fully utilize the potential of RT-PCR technology. The methodology inherently supports the analysis of multiple samples simultaneously, particularly in high-throughput testing environments. Therefore, the correct understanding is that multiple reactions can be conducted, accommodating different templates or conditions.

Options indicating a limitation to one or two reactions do not take into account the flexibility and capacity that current RT-PCR setups provide, especially in molecular diagnostics where the ability to conduct numerous reactions can enhance efficiency and throughput for various analyses.