What is the first step of the Southern Blot process?

The first step of the Southern Blot process involves confirming the presence of DNA. This foundational step is crucial as it allows the researcher to ascertain that there is sufficient DNA to analyze in the subsequent stages of the method. Typically, this step involves the extraction of DNA from cells or tissue samples.

Once the presence of DNA is established, researchers can proceed with gel electrophoresis, which is the next step where the DNA is separated based on size. Following this, DNA is labeled with probes for detection purposes, and finally, the DNA is transferred or blotted onto a nylon membrane for further analysis. Hence, establishing that DNA is present is essential before carrying out the more advanced procedures involved in the Southern Blot technique.