What is the main activity associated with Mung Bean nuclease?

The primary activity of Mung Bean nuclease is the digestion of single-stranded DNA or RNA. This enzyme is derived from the mung bean plant and is particularly effective at degrading nucleic acids that are single-stranded. Its ability to cleave the phosphodiester bonds in the RNA or DNA backbone enables it to shorten or completely digest these single-stranded molecules.

This property makes Mung Bean nuclease useful in various molecular biology applications, including the cleanup of reaction mixtures by degrading leftover single-stranded nucleic acids. It is crucial for ensuring that only double-stranded products remain intact for further analysis or experimentation.

The other activities mentioned in the question, such as digestion of double-stranded RNA and binding to double-stranded DNA, do not accurately represent Mung Bean nuclease's main function. The enzyme does not significantly act on double-stranded RNA nor does it have a role in repairing single-stranded DNA. Understanding the specific enzymatic activities of Mung Bean nuclease is essential for its effective use in laboratory techniques.