Which technique is commonly used for DNA extraction?

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Crude lysis is a technique commonly used for DNA extraction because it effectively breaks open cells to release DNA. This method involves using detergents or enzymes that disrupt the cell membrane, allowing the DNA to be freed from the cell’s nucleus and cytoplasm. The simplicity of crude lysis makes it a widely adopted initial step in the DNA extraction process, especially in laboratory settings where rapid purification is required.

This method is advantageous because it can be performed relatively quickly and does not require sophisticated equipment, making it accessible for various applications, including molecular biology research and diagnostics. Additionally, once the DNA is liberated, it can undergo further purification steps to remove proteins and other cellular debris.

In contrast, techniques like hemolysis are primarily used for breaking down red blood cells, while filtration is not specifically utilized for extracting DNA but rather for separating particles or substances in solutions. Incubation may be involved in certain protocols but it is not a standalone technique for extracting DNA. Hence, crude lysis stands out as the appropriate choice for this process.

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