During hybrid capture, how are RNA:DNA hybrids identified?

The identification of RNA:DNA hybrids in hybrid capture techniques is typically achieved by binding these hybrids to specific antibodies on a microtiter plate. This method utilizes the principle of affinity binding, where antibodies that are designed to recognize the RNA:DNA hybrids are immobilized on the plate. Once the hybrids are formed, they can be captured by these antibodies, allowing for the quantification or analysis of the hybrids.

This technique is advantageous because it enables selective isolation of the RNA:DNA hybrids from other nucleic acids, facilitating further analysis or characterization. The specificity and sensitivity of the antibodies provide a reliable mechanism to ensure that the correct molecules are being identified and measured.

While there are other methods mentioned, such as using a cleavage enzyme or amplifying signals, these approaches do not directly pertain to the identification process in hybrid capture contexts. Cleavage enzymes are typically used in nucleic acid manipulation rather than hybridity assessment, and amplifying the hybridization signal enhances detection rather than serving as the primary identification method. Denaturing cellular DNA doesn't apply in this context, as it refers to the process of separating DNA strands rather than identifying hybrids.

Thus, the use of antibodies in binding to RNA:DNA hybrids provides a clear and targeted approach for their identification during