What distinguishes Exonuclease VII from other exonucleases?

Exonuclease VII is distinguished from other exonucleases by its ability to digest single-stranded DNA from either the 5' or 3' end. This characteristic makes it unique among exonucleases, which typically are specialized for either 5' or 3' ends. Its versatility in processing single-stranded DNA allows it to play a significant role in various molecular biology applications, including DNA repair and manipulation.

In contrast, other options misrepresent the capabilities of Exonuclease VII. Its limitation to only 3' end activity, as stated in one of the choices, does not apply since Exonuclease VII can act on both ends of single-stranded DNA. Additionally, Exonuclease VII is not restricted to double-stranded DNA consumption; therefore, suggesting it only digests double-stranded DNA inaccurately highlights its functionality. Lastly, the synthesis of RNA from DNA templates relates to the action of different enzymes, such as RNA polymerases, rather than exonucleases, further clarifying the specific role of Exonuclease VII in DNA metabolism.