What does a negative template control utilize?

A negative template control is designed to ensure that there is no contamination in the reagents or that the assay does not produce a false positive result. To achieve this, it utilizes a non-target sequence. By including a non-target sequence in the control, the test can effectively demonstrate that any amplification or signal detected during the assay is due to the specific target and not from extraneous sources or contaminants in the reaction mix.

Using a non-target sequence in a negative template control helps to highlight the specificity of the assay, ensuring that the conditions of the test are capable of distinguishing between the intended target and non-target sequences. Thus, if there is a signal produced in the negative control containing a non-target sequence, it indicates a problem with the assay or potential contamination, rather than validating the presence of the target sequence. This is crucial for the integrity and accuracy of molecular diagnostic tests.