What is the result of the probes binding with the invader probe in cleavage-based signal amplification?

In cleavage-based signal amplification, the correct result of the probes binding with the invader probe is the creation of a three-dimensional structure. This structure is a key feature of the molecular mechanism involved in this amplification process.

When the invader probe binds to its complementary target sequence, it creates a specific arrangement that facilitates the interaction with a detector probe. The binding event typically results in a conformational change that allows for the efficient cleavage of the detector probe by a specific enzyme, generating a signal that can be detected and quantified.

This three-dimensional configuration is crucial for the specificity and sensitivity of the assay, ensuring that the amplification occurs only when the correct target is present. The unique arrangement of the probes and the invader thus directly influences the efficiency of the cleavage process, leading to a successful signal amplification.

Other choices reflect different concepts. The release of a detection signal is a result of the amplification process but is not the immediate result of the binding itself. Formation of DNA-RNA hybrids and denaturation of cellular DNA are related to other molecular interactions and processes, but they do not specifically pertain to the result of probes binding in this context. The focus here is primarily on the structural arrangement that occurs during the binding events critical for amplification.