What method is used for isolating DNA that employs a chelating resin?

The method for isolating DNA that employs a chelating resin is effectively captured in the technique often referred to as "boil and isolate." This approach involves the use of a chelating resin, which binds metal ions that may be present in the solution, potentially disrupting protein structures and aiding in the release and stabilization of DNA during the boiling process. The hydrophilic nature of the resin allows for the efficient capture of DNA molecules while leaving behind various cellular contaminants and proteins.

In the context of isolating DNA, utilizing heat can lyse cells and denature proteins, thereby increasing the yield of DNA obtained. The chelating resin enhances the method’s efficiency by sequestering ions that could otherwise interfere with the isolation procedure, ensuring a purer DNA sample.

In contrast, other methods listed such as electrophoresis, filtration, and centrifugation, while useful in various laboratory techniques, do not specifically incorporate the use of a chelating resin for the direct isolation of DNA. Electrophoresis is primarily a separation technique used to analyze nucleic acids, filtration focuses on removing particulate matter, and centrifugation is a physical separation process based on density differences rather than the specific chemistry of DNA isolation with chelation. Thus, the