What type of chromosome spreads are needed for FISH analysis?

For FISH (Fluorescence In Situ Hybridization) analysis, metaphase chromosome spreads or interphase cells are necessary because they provide a suitable context for detecting specific DNA sequences within chromosomes. Metaphase spreads allow for the visualization of chromosomes in their most condensed state, which aids in the identification of specific regions where hybridization occurs. The clear, distinct bands and organization of chromosomes during metaphase facilitate the detection of chromosomal abnormalities or specific gene locations.

Interphase cells are also important because they represent the chromatin's natural state within the cell cycle, which is beneficial for detecting subtler changes in the genetic material that may not be visible during metaphase. This flexibility allows FISH to be employed in various types of samples beyond just those in typical metaphase spreads.

The other options would not provide the necessary clarity needed for effective FISH analysis, as only liver cell spreads or any arbitrary chromosome spreads would not guarantee the optimal nuclear and chromosomal architecture required for accurate application of this technique. Thus, a combination of metaphase spreads and interphase cells is most effective for the desired analysis.