Which is NOT a characteristic of membrane-bound SSP-PCR?

Membrane-bound SSP-PCR, which stands for Sequence-Specific Primer Polymerase Chain Reaction, has distinct characteristics that set it apart from other PCR methods. One key aspect of this technique is the use of a solid phase, which provides a stable environment for the reaction and allows for greater control over the conditions. Therefore, utilizing a solid phase is a defining feature of membrane-bound SSP-PCR.

In addition to this, the method can incorporate advanced technologies such as Luminex, which utilizes color-coded beads for detecting different nucleic acid sequences effectively. This capability enhances the multiplexing ability of the assay, allowing simultaneous detection of multiple targets.

Another essential characteristic is the performance of the assay on immobilized targets, which aids in the specificity of the reaction. This immobilization helps ensure that the amplification occurs only in the presence of the specific target sequences, which is crucial for the method’s reliability and accuracy.

The statement regarding negative readings is not a characteristic of the technique, as SSP-PCR is designed to produce positive results when the target sequence is present. Negative readings may be related to the absence of the target sequence or technique failure, but they do not inherently define the characteristics of membrane-bound SSP-PCR. Thus, the option regarding negative readings correctly identifies something that is