Which of the following is NOT a characteristic of eukaryotic DNA transcription?

The presence of strict promoter sequences is not a characteristic unique to eukaryotic DNA transcription. While eukaryotic transcription does involve specific promoter elements that regulate the binding of RNA polymerase II, the concept of promoter sequences is not exclusive to eukaryotes. Prokaryotic organisms, such as bacteria, also have promoters to initiate transcription, and these sequences can vary significantly. In eukaryotes, the diversity and complexity of transcription factors and regulatory elements allow for a more flexible and nuanced regulation of gene expression, rather than adhering to a strictly defined set of promoter sequences.

In contrast, spliceosomes, which are complexes that mediate the splicing of pre-mRNA, are a hallmark of eukaryotic transcription as they are necessary for the processing of mRNA after transcription. The use of RNA polymerase II is specific to eukaryotes, as it is responsible for transcribing mRNA and some other types of RNA, while the other RNA polymerases cater to different functions. Additionally, eukaryotic transcription typically relies on multiple transcription factors that interact with each other and the RNA polymerase to initiate and regulate transcription efficiently, highlighting the complexity of eukaryotic gene expression.